Samples:
- IgG:
- IGOS 26-28, 30-33, 35-49
- CNTN1/CASPR1 patients
- CIDP78, 17-595, 19-205, 16-047, 20-2, 20-1, R, Vieira, Piffort, 19-216
- Negative control
- Positive control (15-198)
- IgM:
- IGOS 26-28, 30-33, 35-49
- Negative control
DRG NEURONS ICC
- Incubate with patient’s sera in a 24 wells plate: 1 hour 37ºC (prepare the dilution in the wells and then put one coverslip per well)
- For IgG: 2 µl of sera in 300 µl of NG medium
- For IgM: 7,5 µl of sera in 300 µl of NG medium
- Fix with PFA 4 % –> 10 min at room temperature
- Wash 1 time with PBS1x
- Incubate with secondary antibody (1 hour at RT) –> GAH 488 IgG/IgM 1:1000 diluted in Goat serum 5%
- Wash with PBS1x
- Mount with Vectashield
RESULTS
IgG
- IGOS 26 ++
- IGOS 30 ++ / +++
- IGOS 32 +
- IGOS 33 + / ++
- IGOS 38 +
- IGOS 43 +
- IGOS 49 +
- CIDP 78 + / ++
- 17-595 –
- 19-205 –
- 16-047 ++
- 20-2 +
- 20-1 ++ / +++
- R +
- Vieira +
- Piffort ++ / +++
- 19-216 +
IgM
- 26 ++
- 28 +
- 30 +
- 31 +
- 33 +
- 36 +
- 38 + / ++
- 40 +
- 43-49 repetir
