20190515: ICC NF155 IgG in CIDP patients (Birminham)

Objective: To test CIDP patients for IgG positivity towards NF155.

Samples: 189-164, 19-165, 19-166, 19-167, 19-168, negative control, positive control (LAC).

ICC NF155:

  • Transfection date: 2019.05.09
  • Blocking solution: 5% goat serum in PBS.
  • Patient’s sera diluted 1/100 for IgG with 5% goat serum in PBS.
  • Primary antibody: AF3235 diluted 1/1000.
  • Secondary antibodies: GAC488 + GAH 594 IgG (1/1000).
  • Vectashield mounting medium.

RESULTS: 

All samples wew negative.

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