Objective: To test CIDP patients for IgG positivity towards NF155.
Samples: 189-164, 19-165, 19-166, 19-167, 19-168, negative control, positive control (LAC).
ICC NF155:
- Transfection date: 2019.05.09
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/100 for IgG with 5% goat serum in PBS.
- Primary antibody: AF3235 diluted 1/1000.
- Secondary antibodies: GAC488 + GAH 594 IgG (1/1000).
- Vectashield mounting medium.
RESULTS:
All samples wew negative.