Objective: To test CMT patients for IgM positivity towards NF186 and NF140.
Samples: For NF140: 87-111, negative control. For NF186: 83-107, negative control.
ICC NF186/NF140:
- Transfection date: 2019.05.01
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/100 for IgG with 5% goat serum in PBS.
- Primary antibody: AF3235 diluted 1/1000.
- Secondary antibodies: GAC488 + GAH 594 IgM (1/1000).
- Vectashield mounting medium.
RESULTS: All patients were negative. We have to repeat sample 106-107-108 for NF140.
