20190506: ICC NF186 and NF140 IgM in CMT patients

Objective: To test CMT patients for IgM positivity towards NF186 and NF140.

Samples: 58-82, negative control.

ICC NF186/NF140:

  • Transfection date: 2019.05.01
  • Blocking solution: 5% goat serum in PBS.
  • Patient’s sera diluted 1/100 for IgG with 5% goat serum in PBS.
  • Primary antibody: AF3235 diluted 1/1000.
  • Secondary antibodies: GAC488 + GAH 594 IgM (1/1000).
  • Vectashield mounting medium.

RESULTS: All samples were negative. 

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