ICC CNTN1:
- Blocking solution: Rabbit serum diluted 1/40 in PBS.
- Patient’s sera diluted 1/100
- Primary antibody (anti-CNTN1 AF904) diluted 1/1000.
- Secondary antibodies: RAG 488 + RAH 594 IgG (1/1000)
- Vectashield mounting medium.
Samples and result:
| Sample | Result | Sample | Result |
| 19-059 | – | 19-166 | – |
| 19-060 | – | 19-167 | – |
| 19-061 | – | 19-168 | – |
| 19-164 | – | Neg. control | – |
| 19-165 | – | Pos. control 15-198 | + |
ICC CNTN1-CASPR1:
- Blocking solution: Goat serum 5 % diluted in PBS.
- Patient’s sera diluted 1/100
- Primary antibody (anti-CASPR1) diluted 1/250.
- Secondary antibodies: GAR 488 + GAH 594 IgG (1/1000)
- Vectashield mounting medium.
Samples and result:
| Sample | Result | Sample | Result |
| 19-061 | – | 19-167 | – |
| 19-164 | – | 19-168 | – |
| 19-165 | – | Neg. control | – |
| 19-166 | – | Pos. control 19-003 | + |
