Detection of IgG positivity towards NF186 in CIDP patients from Bochum (CIDP 62-89).
Samples:
- CIDP patients sera 62-89
- positive control
- negative control
Protocol:
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/100 for IgG with 5% goat serum in PBS.
- Primary antibody: AF3235 diluted 1/1000.
- Secondary antibodies: GAC488 + GAH 594 IgM (1/1000).
- Vectashield mounting medium 4µl/Coverslip
Results:
- CIDP62 neg.
- CIDP63 neg.
- CIDP64 neg.
- CIDP65 neg.
- CIDP66 neg.
- CIDP67 neg.
- CIDP68 neg.
- CIDP69 neg.
- CIDP73 neg.
- CIDP74 neg.
- CIDP75 neg.
- CIDP76 neg.
- CIDP77 neg.
- CIDP78 neg.
- CIDP80 neg.
- CIDP81 neg.
- CIDP82 neg.
- CIDP83 neg.
- CIDP84 neg.
- CIDP85 neg.
- CIDP86 neg.
- CIDP87 neg.
- CIDP88 neg.
- CIDP89 neg.
