Samples:
| Sample | Result NF140 | Result NF186 | Sample | Result NF140 | Result NF186 |
| 19-11 | – | – | 19-19 | – | – |
| 19-12 | – | – | 19-27 | – | – |
| 19-13 | – | – | 19-28 | – | – |
| 19-14 | – | – | 19-29 | – | – |
| 19-15 | – | – | 19-30 | – | – |
| 19-16 | – | – | 19-31 | weak + | – |
| 19-17 | – | – | Neg. control | – | – |
| 19-18 | – | – | Pos. control (16-069) | + | + |
ICC NF155
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/100 for IgG with 5% goat serum in PBS.
- Primary antibody: AF3235 diluted 1/1000.
- Secondary antibodies: GAC488 + GAH 594 IgG (1/1000).
- Vectashield mounting medium.
RESULTS:
All samples are negative.
