Objectives: To test CIDP patients for IgG positivity towards CNTN1.
Samples: From 24 to 63.
| 24 | - |
|---|---|
| 25 | - |
| 26 | - |
| 27 | - |
| 28 | - |
| 29 | - |
| 30 | - |
| 31 | - |
| 32 | - |
| 33 | - |
| 34 | - |
| 35 | - |
| 36 | - |
| 37 | - |
| 38 | - |
| 39 | - |
| 40 | - |
| 41 | - |
| 42 | - |
| 43 | - |
| 44 | - |
| 45 | - |
| 46 | - |
| 47 | - |
| 48 | |
| 49 | - |
| 50 | Repetir |
| 51 | Repetir |
| 52 | - |
| 53 | - |
| 54 | - |
| 55 | - |
| 56 | - |
| 57 | - |
| 58 | - |
| 59 | - |
| 60 | - |
| 61 | - |
| 62 | - |
| 63 | - |
| CONTROL- | - |
| CONTROL+ | + |
CTN1 ICC:
- Blocking solution: Rabbit serum diluted 1/40 in PBS.
- Patient’s sera diluted 1/100 for IgG with rabbit serum 1/40.
- Primary antibody: AF904 diluted 1/1000.
- Secondary antibodies: RAG 488 + RAH 594 IgM (1/1000)
- Vectashield mounting medium.
Results: All samples were negative. We have to repeat sample 50 and 51 because there weren’t enought HEK cells in the plates.
