Objectives: To test CIDP patients for IgG positivity towards CNTN1 and CNTN1/CASPR1.
Samples:
| CODE | RESULT CNTN1 | RESULT Doble |
|---|---|---|
| 0 | - | - |
| 1 | ? | - |
| 2 | - | - |
| 3 | ? | - |
| 4 | - | - |
| 5 | - | - |
| 6 | - | - |
| 7 | - | - |
| 8 | - | - |
| 9 | - | - |
| 10 | - | - |
| 11 | - | - |
| 12 | - | - |
| 13 | - | - |
| 14 | - | - |
| 15 | - | - |
| 16 | ? | - |
| 17 | - | - |
| 18 | - | - |
| 19 | - | - |
| 20 | - | - |
| 21 | - | - |
| 22 | - | - |
| CTRL + ( 15-198) | + | + |
| CTRL - | - | - |
CTN1 ICC:
- Blocking solution: Rabbit serum diluted 1/40 in PBS.
- Patient’s sera diluted 1/100 for IgG with rabbit serum 1/40.
- Primary antibody: AF904 diluted 1/1000.
- Secondary antibodies: RAG 488 + RAH 594 IgM (1/1000)
- Vectashield mounting medium.
CNTN1/CASPR ICC:
- Blocking solution: Goat serum diluted at 5% in PBS.
- Patient’s sera diluted 1/100 for IgG with goat serum 5% in PBS.
- Primary antibody: Ab 1336341 diluted 1/250
- Secondary antibodies: GAR488 + GAH 594 IgG (1/1000)
- Vectashield mounting medium.
Results: Patient 1, 3 and 16 have a doubtful staining for IgG towards CNTN1. We are going to perform an ELISA with these 3 patients.
