Objectives: To test Multiple Sclerosis patients for IgG positivity towards ANO2
Patients: 145 to 167 (from the Multiple Sclerosis sera collection) + negative control
ANO2 ICC:
- HEKs 293 Cells Transfection: 12.03.2018
- Blocking solution: Goat Serum diluted 5% in PBS.
- Patient’s sera diluted 1/100 with 5 % Goat Serum
- Primary antibody: ANO2 (TA316534 OriGene) diluted 1/500.
- Secondary antibodies: GAR 488 + GAH 594 IgG (1/1000)
- Vectashield mounting medium.
Resultado: 145 positivo débil. 153 positivo (comprovar haciendo ICC sin Ac comercial)
