20180129: ICC GDAP1L1 CIDP patients

Objectives: To test CIDP patients for IgG positivity towards GDAP1L1.

Patients:

NUMBERCODE
118-524
218-525
318-526
418-527
518-528
618-529
718-530
818-531
918-532
1018-533
1118-534
1218-535
1318-536
1418-537
1518-538
1618-539
1718-540
1818-541
1918-542
2018-543
2118-544
2218-545
2318-546
24CTRL -

GDAP1L1 ICC:

  • HEKs 293 Cells Transfection: 2018.01.22
  • Blocking solution: 5% goat serum in PBS.
  • Patient’s sera diluted 1/100 for IgG with 5% goat serum in PBS.
  • Primary antibody: Mouse GDAP1L1 diluted 1/200
  • Secondary antibodies: GAM488 (1/500) + GAH594 IgG (1/500).
  • Vectashield mounting medium

Results: All samples were negative.

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