Objectives: To test CIDP patients for IgM positivity towards NF155.
Patients:
| NUMBER | CODE |
|---|---|
| 1 | 18-502 |
| 2 | 18-503 |
| 3 | 18-504 |
| 4 | 18-505 |
| 5 | 18-506 |
| 6 | 18-507 |
| 7 | 18-508 |
| 8 | 18-572 |
| 9 | 18-573 |
| 10 | 18-574 |
| 11 | 18-575 |
| 12 | 18-576 |
| 13 | 18-577 |
| 14 | 18-578 |
| 15 | 18-579 (PBS) |
| 16 | 18-580 (PBS) |
| 17 | CTRL - |
| 18 | 18-579 (AGUA DEST) |
| 19 | 18-580 (AGUA DEST) |
NF155 ICC:
- HEKs 293 Cells Transfection: 2018.01.16
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/10 for IgM with 5% goat serum in PBS.
- Primary antibody: AF3235 diluted 1/1000.
- Secondary antibodies: GAH 594 IgM (1/1000) + GAC 488 (1/1000).
- Vectashield mounting medium
Results: All samples were negative.
