Primary culture Schwann cell IgG ICC is performed to test Zika-GBS patients’ staining pattern.
PATIENTS
The following patients were analyzed by IgG:
SUEROS ZIKA | |
---|---|
73 | Z014-4 16/04/16 |
74 | Z011-4 16/04/16 |
75 | Z056-4 14/05/16 |
76 | Z023-4 16/04/16 |
77 | Z032-4 16/04/16 |
78 | Z026-4 16/04/16 |
79 | Z038-4 16/04/16 |
80 | Z024-4 16/04/16 |
81 | Z043-4 14/05/16 |
82 | Z059-4 14/05/16 |
83 | Z041-4 14/05/16 |
84 | Z044-4 14/05/16 |
85 | Z040-4 14/05/16 |
86 | Z057-4 14/05/16 |
87 | Z004-4 16/04/16 |
88 | Z031-5 16/04/16 |
89 | Z019-4 16/04/16 |
90 | Z013-4 16/0416 |
91 | Z062-4 14/05/16 |
92 | Z065-4 16/05/16 |
93 | Z061-4 14/05/16 |
94 | Z060-4 28/06/16 |
95 | Z055-4 14/05/16 |
96 | Z034-5 16/04/16 |
97 | Z001-4 16/04/16 |
98 | Z035-4 16/04/16 |
99 | Z058-4 16/04/16 |
100 | Z029-4 16/04/16 |
101 | Z018-4 16/04/16 |
102 | Z063-4 14/05/16 |
103 | Z006-4 15/04/16 |
104 | Z052-4 14/05/16 |
105 | Z030-4 16/04/16 |
106 | Z010-4 16/04/16 |
107 | Z039-4 14/05/16 |
108 | Z005-4 16/04/16 |
109 | Z036-4 16/04/16 |
110 | Z027-4 16/04/16 |
111 | Z054-4 14/05/16 |
112 | Z016-4 16/04/16 |
113 | Z028-4 16/04/16 |
114 | Z015-4 16/04/16 |
115 | Z053-5 14/05/16 |
116 | Z033-4 16/04/16 |
Materials
- Rat primary culture of Schwann grown in coverslips. Fixed with PFA 4% and blocked with GOAT 5% in PBS.
- PBS
- Blocking: GOAT 5% in PBS
- Patient’s sera diluted 1/100 with GOAT 5% in PBS
- Secondary Abs: GAH 488 IgG (1/1000) (box 25)
- Vectashield mounting medium
RAT PRIMARY CULTURE SCHWANN CELL ICC
- Primary culture Schwann cells grown in coverslips were fixed and blocked with GOAT 5% in PBS and frozen at -80ºC.
- Thaw these cells with a little GOAT 5% in PBS
- Perform ICC:
- Add patient’s sera 1/100 1h at RT.
- Wash 3 times with PBS.
- Add secondary Ab GAH 488 IgG (1/1000) each and incubate 1h at RT.
- Wash 3 times with PBS.
- Mount with Vectashield mounting medium.
RESULTS
All samples are negative except for numbers 87,89,104 & 105 which faintly stain (+) Schwann cells.