Primary culture Schwann cell IgG ICC is performed to test Zika-GBS patients’ staining pattern.
PATIENTS
The following patients were analyzed by IgG:
SUEROS ZIKA | |
---|---|
1 | ZN001A-4 16/04/16 |
2 | ZN014A-5 16/04/16 |
3 | ZN009D-4 14/05/16 |
4 | ZN011A-5 16/04/16 |
5 | ZN004A-5 16/04/16 |
6 | ZN003A-5 16/04/16 |
7 | ZN039C-4 14/05/16 |
8 | ZN012B-4 16/04/16 |
9 | ZN013A-5 16/04/16 |
10 | ZN022A-4 16/04/16 |
11 | ZN005A-5 16/04/16 |
12 | ZN027A-4 16/04/16 |
13 | ZN018A-5 16/04/16 |
14 | ZN009B-5 16/04/16 |
15 | ZN010A-4 16/04/16 |
16 | ZN025B-4 16/04/16 |
17 | ZN041A-5 14/05/16 |
18 | ZN008A-5 16/04/16 |
19 | ZN019A-5 16/04/16 |
20 | ZN047A-6 14/05/16 |
21 | ZN007A-4 16/04/16 |
22 | ZN052A-4 14/05/16 |
23 | ZN056A-5 28/06/16 |
24 | ZN009E-4 14/05/16 |
25 | ZN023A-6 16/04/16 |
26 | N009A-4 16/04/16 |
27 | ZN002A-5 16/04/16 |
28 | ZN044A-5 14/05/16 |
29 | ZN041B-4 14/05/16 |
30 | ZN020A-5 16/04/16 |
31 | ZN009C-5 16/04/16 |
32 | ZN058A-5 28/06/16 |
33 | ZN012A-4 13/04/16 |
34 | ZN046A-5 14/05/16 |
35 | ZN012C-4 14/05/16 |
36 | ZN040A-5 14/05/16 |
37 | ZN039B-4 14/05/16 |
38 | ZN060E-3 13/07/16 |
39 | ZN060F-3 13/07/16 |
40 | ZN016A-4 16/04/16 |
41 | ZN043A-5 14/05/16 |
42 | ZN060B-4 28/06/16 |
43 | ZN057A-5 28/06/16 |
44 | ZN015A-4 16/04/16 |
45 | ZN048A-5 14/05/16 |
46 | ZN017A-5 16/04/16 |
47 | ZN026A-5 16/04/16 |
48 | ZN025A-4 16/04/16 |
49 | ZN060D-4 28/06/16 |
50 | ZN060C-4 28/06/16 |
51 | ZN039A-5 14/05/16 |
52 | ZN022B-4 16/04/16 |
53 | ZN040B-4 14/05/16 |
54 | ZN061A-6 28/06/16 |
55 | ZN060A-3 13/07/16 |
56 | ZN059A-4 28/06/16 |
57 | ZN006A-6 16/04/16 |
58 | ZN055A-6 28/06/16 |
59 | ZN045A-5 14/05/16 |
60 | Z008-4 16/04/16 |
61 | Z003-4 16/04/16 |
62 | Z002-4 16/04/16 |
63 | Z042-4 14/05/16 |
64 | Z022-4 16/04/16 |
65 | Z017-4 16/04/16 |
66 | Z025-4 16/04/16 |
67 | Z012-4 16/04/16 |
68 | Z009-4 16/04/16 |
69 | Z007-4 16/04/16 |
70 | Z037-4 16/04/16 |
71 | Z021-4 16/04/16 |
72 | Z020-4 16/04/16 |
Materials
- Rat primary culture of Schwann grown in coverslips. Fixed with PFA 4% and blocked with GOAT 5% in PBS.
- PBS
- Blocking: GOAT 5% in PBS
- Patient’s sera diluted 1/100 with GOAT 5% in PBS
- Secondary Abs: GAH 488 IgG (1/1000) (box 25)
- Vectashield mounting medium
RAT PRIMARY CULTURE SCHWANN CELL ICC
- Primary culture Schwann cells grown in coverslips were fixed and blocked with GOAT 5% in PBS and frozen at -80ºC.
- Thaw these cells with a little GOAT 5% in PBS
- Perform ICC:
- Add patient’s sera 1/100 1h at RT.
- Wash 3 times with PBS.
- Add secondary Ab GAH 488 IgG (1/1000) each and incubate 1h at RT.
- Wash 3 times with PBS.
- Mount with Vectashield mounting medium.
RESULTS
All samples are negative except for number 15 and number 67, which faintly stain(+) Schwann cells