Objective To verify that what we saw are nodes, we will mark this rat skin cuts with CASPR and neurofascina. We aim to see colocalizing…
Objective In the last experiment we can see nodes very well, even better than teasing technique. So, we try to do it with sera. We’ll…
Objective Is a positive control for 20140305 rat nerve IHC free floating Materials Paraformaldehyde Sucrose 30% + azide 15mM / PBS 24-well plate PBS Cryostat…
Objective We’ll do the same experiment but with rat nerve instead of skin trying to verify the technique because we know how nerves looks like.…
Objective Set up IHC free-floating technique with glabrous rat skin. We’ll see neurofilament and try CASPR to detect nodes. Materials see rat skin IHC 3 Mouse-anti-CASPR Procedure…
Objective Set up IHC free-floating technique with glabrous rat skin. We’ll see neurofilament and MBP to detect myelin fibers. Materials see rat skin IHC 2…
Objective Set up a new technique, rat skin IHC, with changes respect 1: Change 1: filter secondary Ab Change 2: permeability cells with PBS-triton0,3% …
Objective Set up a new technique, rat skin IHC Materials Paraformaldehyde Sucrose 30% + azide 15mM / PBS 24-well plate PBS Cryostat (OTC, criomold) Goat…