Objective We need transfected HEK grown in coverslips to test patients sera against CNTN. We’ll do 2 plates for CNTN Materials 60mm plate coverslips Poly-D-Lisine…
Objective We need transfected HEK grown in coverslips to test patients sera against LRP4 and CNTN. We’ll do 7 plates for LRP4 Materials 60mm plate coverslips…
Objective The aim of this experiment is to decrease the background of the last experiment. We’ll try not to permeabilize. We’ll try MBP 1:100 and…
Objective We need transfected HEK grown in coverslips to test patients sera against LRP4 and CNTN. We’ll do 6 plates for LRP4 and 2 for CNTN…
Objective Set up IHC free-floating technique with glabrous rat skin. We saw positive marks for MBP but too much background, so we’ll try with blocking…
Objective The aim of this experiment is to screen seronegative patients from MG (SNMG) with LRP4 protein. The purpose is to determine the frequency of…
Objective The aim of this experiment is to screen seronegative patients from MG (SNMG) with LRP4 protein. The purpose is to determine the frequency of…
Objective Amplify DNA to transfect HEK (CNTN-GFP and NF155) Materials One Shoot TOP10 Chemically competent E. coli transfected with NF155 (pCMV6-NF155-DDKMyc (Human)) One Shoot TOP10…
Objective The aim of this experiment is to screen seronegative patients from MG (SNMG) with LRP4 protein. The purpose is to determine the frequency of…
Objective We want HEK cels transfected with NFASC, CNTN and LRP4. Then we corroborate the transfection using antibodies against each protein. We want 3 plates…