2024.02.08 – ICC CNTN1 – Muestras Austria VIVAS

Muestras:

  1. C18.1 – (C166/13)
  2. C19.1 – (C3097/18)
  3. C22.1 – (c7006/18)
  4. Control positivo
  5. Control negativo

Protocolo:

  1. Incubar 1 hora a 37 ºC el suero 1/100
  2. 3 lavados PBS1x
  3. Bloquear con rabbit serum 1/40 1 hora
  4. Incubar 1h con anticuerpo primario — Anti- CNTN1 1/1000
  5. 3 lavados con PBS1x
  6. Incubar 1h con Ac secundario diluido RAG488 + RAH594 1/1000
  7. 3 lavados  con PBS 1x

Resultados:

  1. C18.1 – (C166/13) — negativo
  2. C19.1 – (C3097/18)– negativo
  3. C22.1 – (c7006/18)– positivo
  4. Control positivo– positivo
  5. Control negativo– negativo
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