OBJECTIVES:
- To detect antibodies against CNTN1 in CIDP patients from a clinical trial(ICQ).
SAMPLES:
- For CNTN1: 5,8,9,10,11,16-34, positive control.
ICC CNTN1:
Blocking solution: Rabbit serum in PBS.
- Patient’s sera diluted 1/100 in rabbit sèrum in PBS.
- Primary antibody: anti-CNTN1 diluted 1/1000.
- Secondary antibodies: RAG 488 + RAH 594 (1/1000)
- Vectashield mounting medium.
RESULTS: All samples were neagtive.