20210426: ICC NF140/NF155/NF186 and CNTN1 in Guillain-Barré patients after SARS-CoV-2 infection

Objective: To detect antibodies against NF155, nodal neurofascins (NF140, NF186) and CNTN1.

Samples: 21-2-0768, 21-2-0769, 21-2-0770, 21-2-0771, 21-2-0772, 21-2-0773, 21-2-0774, 21-2-0775, 21-2-0776.

Immunocytochemistry for CNTN1:

Blocking solution: Rabbit serum 1/40in PBS.

• Incubate for 1 hour patient’s sera diluted 1/100 in rabbit serum in PBS.
• Primary antibody: anti-CNTN1 diluted 1/1000.
• Secondary antibodies: RAG 488 + RAH 594 (1/1000)
• Vectashield mounting medium.

Immunocytochemistry for NF155/NF140/NF186:

Blocking solution: 5% goat serum in PBS.

• Patient’s sera diluted 1/100 in 5% goat serum in PBS.
• Primary antibody: anti-NF diluted 1/1000.
• Secondary antibodies: GAC488 + GAH 594 (1/1000).
• Vectashield mounting medium

Results: All samples were negative.