Samples:
- 1. negative control
- 2. Regine
- 3. Piffort
- 4. Vieira
- 5. 19-186
Protocol:
- Blocking solution: goat 5% in PBS
- Patients’ sera diluted 1:100 ( goat 5% in PBS)
- Ac anti-Caspr1 diluted 1/250
- GAH 594 IgG and GAR 488 IgG diluted 1/1000
- Vectashield mounting medium
Results:
- 1. negative control: NEGATIVE
- 2. Regine: MILD POSITIVE
- 3. Piffort: POSITIVE ++
- 4. Vieira: NEGATIVE
- 5. 19-186: NEGATIVE ¿?
