Objective: To test CMT patients for IgG positivity towards NF186 and NF140.
Samples: 106-116, positive control, negative control.
ICC NF140/NF186:
- Transfection date: 2019.05.09
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/100 with 5% goat serum in PBS.
- Primary antibody: AF3235 diluted 1/1000.
- Secondary antibodies: GAC488 + GAH 594 IgG (1/1000).
- Vectashield mounting medium.
RESULTS: All patients were negative.
