20190306: ICC CNTN1 in CMT (Santander) and CIDP samples (Birmingham)

Objectives: To test CIDP and CMT patients for IgG positivity towards CNTN1.

Samples: CIDP – 19-027, 19-028, 19-029. CMT: 58-81, CTRL – 11, CTRL + (15-198).

CNTN1 ICC:

• Blocking solution: Rabbit serum diluted 1/40 in PBS.
• Patient’s sera diluted 1/100 for IgG with rabbit serum 1/40.
• Primary antibody: AF904 diluted 1/1000.
• Secondary antibodies: RAG 488 + RAH 594 IgG (1/1000)
• Vectashield mounting medium.

RESULTS: All samples were negative.