Objectives: To test CIDP patients for IgG positivity towards CNTN1/CASPR1 and NF186.
Samples: 46-69
Trasfection: 26.07.18
| SAMPLE | CNTN1/CASPR | NF186 |
|---|---|---|
| 46 | - | - |
| 47 | Repetir | - |
| 48 | - | Repetir |
| 49 | - | - |
| 50 | - | - |
| 51 | - | - |
| 52 | - | - |
| 53 | - | - |
| 54 | - | - |
| 55 | - | - |
| 56 | - | - |
| 57 | - | - |
| 58 | - | - |
| 59 | - | - |
| 60 | - | - |
| 61 | - | - |
| 62 | - | - |
| 63 | - | - |
| 64 | - | - |
| 65 | - | - |
| 66 | - | - |
| 67 | - | - |
| 68 | - | - |
| 69 | - | - |
| CONTROL- | - | - |
| CONTROL+ | + | + |
CNTN1/CASPR ICC:
- Blocking solution: Goat serum diluted at 5% in PBS.
- Patient’s sera diluted 1/100 for IgG with goat serum 5% in PBS.
- Primary antibody: Ab 1336341 diluted 1/250
- Secondary antibodies: GAR488 + GAH 594 IgG (1/1000)
- Vectashield mounting medium
NF186 ICC:
- HEKs 293 Cells Transfection: 2018.07.26
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/100 for IgG with 5% goat serum in PBS.
- Primary antibody: AF3235 diluted 1/1000.
- Secondary antibodies: For IgG: GAC 488 (1/1000) + GAH 594 IgG (1/1000)
- Vectashield mounting medium.
Results: All samples were negative. We have to repeat sample 47 for CNTN1/CASPR and sample 48 for NF 186 because there were not enough transfected cells in the plate
