20180618: IHQ – Peripheral nerve monkey Euroimmun (prueba)

Patients:

1. Positive control (kit)
2. Negative control (kit)
3. 17-595 –> O’Nions
4. 15-198 –> CNTN1 positive
5. 15-195–> NF155 positive

IHC: 

• Comercial tissue sample: FA 1120-1005 (Euroimmun)
• Patient’s sera diluted 1/10 for IgM with PBS-tween –> put 30 microl of the dilution in every well of the reactives support. Then, put the slide on.
• Wash the slide 2 times with PBS-tween (with shaking)
• Put 25 microl of the secondary antibody (an anti-human conjugate against IgG, IgA and IgM) in every well of the reactives support. Then, put the slide on.
• Wash the slide 2 times with PBS-tween (with shaking)

Results: there are no differences between the different samples (only between the negative control and the other samples).