Objectives: To test CIDP patients for IgG positivity towards GDAP1L1.
Patients:
| NUMBER | CODE |
|---|---|
| 1 | 18-513 |
| 2 | 18-514 |
| 3 | 18-515 |
| 4 | 18-516 |
| 5 | 18-517 |
| 6 | 18-518 |
| 7 | 18-519 |
| 8 | 18-520 |
| 9 | 18-521 |
| 10 | 18-522 |
| 11 | 18-523 |
GDAP1L1 ICC:
- HEKs 293 Cells Transfection: 2018.01.22
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/100 for IgG with 5% goat serum in PBS.
- Primary antibody: Mouse GDAP1L1 diluted 1/200
- Secondary antibodies: GAM488 (1/500) + GAH594 IgG (1/500).
- Vectashield mounting medium
Results: All samples were negative.
