Objectives
To obtain more DNA to perform a transfection.
Materials
see last maxi
- Plasmids from Gisela (MPZ, PMP2, MOG)
- Qiagen HiSpeed plasmid maxi kit
Methods
see last maxi
Results & conclusions
MPZ: Cells grew in bacterial plates (in both antibiotics); but the maxi didn’t grow well with kanamicine. We need to repeat it.
PMP2: 0.4ug/ul
MOG: 0.3 ug/ul
