Samples: Positive control (NF155+) 15-226 Negative control Both samples are tested in pre-fixed tissue (with PFA 4 %) PROTOCOL: Defrost the slides Use the marker…
Samples: 15-198 (CNTN1+) Negative control Nerve lysate: Clean and disgregate the tissue as much as possible Grind (crush) the nerves with a Potter Homogenizer (in…
Samples: 16-134 Positive control (CNTN1+) 15-198 Negative control Positive control (NF155+) 15-226 Negative control 1-3: done in fresh teased fibers (not fixed with PFA 4%);…
OBJECTIVES: To confirm positive result on ICC. SAMPLES: 23, 24, CTRL – and CTRL + (CNTN1: 15-198) PROTOCOL: Fixation: in acetone 10 min at RT Wash…
SAMPLES: 23, 24, CTRL – and CTRL + (CNTN1: 15-198) PROTOCOL: Fixation: in acetone 10 min at RT Wash 2 times with PBS (5 min)…
Samples: 153 PROTOCOL: Fixation: in acetone 10 min at RT Wash 2 times with PBS (5 min) with agitation Use the marker(DakoPen) to highlight the…
Samples: OP47 (Can Ruti): 18-673 (ICQ) Mª Dolors Gili ripoll: 18-192 (Ser. Immuno) Positive control (15-198) Negative control PROTOCOL Fixation: in acetone 10 min at…
Dia 12.06.2018: Després de treure el nervi ciàtic del porc i netejar-lo, els grups de fibres nervioses s’han fixat en PFA 4 % , o…
SAMPLES We will test the Zika samples from Dr. Willison’s lab and 4 samples from our ICQ database. ICQ samples: 18-612 Guillem Mateo Nieto 18-613…
To test whether 4% PFA -fixed (10 min) and 4% PFA-fixed (10 min)+ 5% Goat-blocked (1 hour) teased swine sciatic frozen nerves perform better in IHC experiments when compared to straight-frozen…