OBJECTIVE To set-up a sorting protocol to isolate the B-cell subset responsible of producing pathogenic antibodies in neuropathies. The whole process is copied/adapted from Chihara…
OBJECTIVE Set up Schwann cell ICC to test the presence of antibodies against Schwann cells in CIDP patients MATERIALS Schwann cell line (P3), Innoprot PFA…
OBJECTIVE The same as previous ones. In this case changing the CD3 color (BD, CD3-PE_Cy7 clone SK7) MATERIALS Same as previous experiments, except the CD3.…
OBJECTIVE Set up FACS rotocol to characterize B cell subpopulations. PROCEDURE Isolate PBMCs from healthy donor with Ficoll-Paque (followed Ficoll protocol) Washed 2x with PBS…
OBJECTIVE Set up FACS rotocol to characterize B cell subpopulations. PROCEDURE Isolate PBMCs from healthy donor with Ficoll-Paque (followed Ficoll protocol) Washed 2x with PBS…