OBJECTIVE To evaluate the amount of B cells producing IL10 To analyze if they all are plasma cells as previously reported To compare 2 different…
OBJECTIVE To test 3 new patients with CIDP suspicion for anti-CNTN1 -NF155 antibodies MATERIALS NF155-HEK cell line, grown in coverslips and frozen (see previous experiment)…
OBJECTIVE To create a stable-transfectant that expresses NF155 constitutively MATERIALS – pCMV6-NF155-myc-DDK purchased from Origene. – Geneticin/G418 Life Technologies – Chicken polyclonal anti-NF – GAC…
OBJECTIVE To set up a contactin-1 ELISA to titrate antiCNTN1+ patients’ sera. MATERIALS Nunc MaxiSorp ELISA plates HVH1 patient sera Recombinant Human CNTN1 protein (Sino…
Yesterday plasma exchange on NFASC 155+ patient Jaume Marcet Lucas. Stored O/N at 4ºc and today proceeded to aliquoting. We had: Around 600mL concentrated plasma…
OBJECTIVE To test reactivity against DRG neurons in patients with CIDP MATERIALS DRG neurons plated on coverslips the on 12.06.2013. We followed Liu et al…
OBJECTIVE Test patients that needed to be repeated from last experiment and check reactivity over Schwann cells fixed with PFA of those patients testing positive…
OBJECTIVE Test patients and controls after setting up the assay PROCEDURE We only perform live staining because it seems to be more sensitive according to…
OBJECTIVE To sort plasmablasts, naive B cells and memory B cells to see if we can detect antibodies against CNTN1/CASPR1 complex in these subsets. MATERIALS…
OBJECTIVE To sort plasmablasts, naive B cells and memory B cells to see if we can detect antibodies against NF155 in these subsets. MATERIALS Same…