IHC Teased nerve fiber (Swine)
CNTN1+ 1/100 | S100 Rabbit 1/100 | GAR 488 + GAH 594 IgG 1/1000 | Mielina y pocos paranodos |
CIDP 100 1/100 | S100 Rabbit 1/100 | GAR 488 + GAH 594 IgG 1/1000 | Mielina limpia. No veo “cajal bands” |
HC Peripheral nerve sections (Monkey)
1 | CIDP 100 1/10 | S100 Rabbit 1/40 | GAR 594 + GAH IgG 488 1/1000 | Fondo, y neurilema? | |
2 | CIDP 100 1/10 | S100 Rabbit 1/40 | GAR 594 + GAH IgM 488 1/1000 | Mielina | |
3 | CIDP 100 1/10 | S100 Rabbit 1/40 | Kappa 1/500 | GAR 488 1/1000 + strep 595* | Mielina |
4 | CIDP 100 1/10 | S100 Rabbit 1/40 | Lambda 1/500 | GAR 488 1/1000 + strep 595* | Negativo (axones) |
*ANTICUERPOS GOAT STREPTAVIDINA 594 32356 INVITROGEN (PREDILUCIÓN 1uL/200uL —> SEGUNDA DILUCIÓN 50UL/0.5mL) 1h
VER FOTO DE RESULTADOS