2023.08.08 IHC Monkey peripheral nerve sections CIDP data base

Protocolo: Monkey peripheral nerve slides

  • Bloquear 30 min – 1h con Goat serum 5%
  • Incubar 1h con 40 ul de suero diluído 1/10 en Goat serum 5% (4 ul suero + 36 ul goat 5%)
  • 3 lavados con PBS1x
  • Incubar 1h con 30-40 ul de Ac. secundarios : GAH monkey absorbed IgG 488 + GAH IgM 594 diluídos 1/500 en goat serum 5%
  • 3 lavados con PBS1x
  • Montar con fluoromount

RESULTS

IgGIgM
NodoMielinaAxones gruesosAxones finosSchwann amielinicasObservaciones IgGPATRON IgGNodoMielinaAxones gruesosAxones finosSchwann amielinicasotrosPATRON IgM
CIDP 10300110nucelosNegativo01 medano calibre000Mielina patrón RAYAS
CIDP 10400001citoplasma SCcitoplasma SC mielnicas y amielnicas00000fibrosNegativo
CIDP 10502000Mielina 2+010000Mielina 1+
CIDP 10600001fondoamielnicas000000Negativo
CIDP 10700200Axones 2002000Axones
CIDP 10800101Axon y amielinica000000Negativo
CIDP 10900101Axon y amielinica000000Negativo
CIDP 11000119Axones G y F000000Negativo
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