Objective: To investigate Monkey’s Peripheral nerve IHC for IgG staining paterns of Amyotrofic neuralgia patients with NCAM Ab, a colocalization with NCAM commercial mAb.
Samples: 21-2-0107, 21-2-0108, 21-2-0109, 21-2-0110, 21-2-0111.
IHQ protolocol:
- We used commercial slides from Inova (peripheral nerve)
- Blocking with Goat Serum 5% in PBS (blocking solution). Incubate 1 hour at RT in a humid chamber.
- Incubate 35ul of sample diluated 1/20 (IgG) or 1/10 (IgM) in blocking solution 1h.
- Wash 2-3 times with PBS 1x.
- Incubate with anti-N-CAM commercial mAb (1/50) 1h.
- Wash 2-3 times with PBS 1x.
- Secondary Ab GAH monkey absorbed 488 and GAM594 (1/500) for 1 hour at RT.
- Wash 3 times with PBS 1x.
- Mount with Fluoromount mounting medium.
Results: the results were here.
Next to the image is indicated the magnification of the microscope lens (20x).
IgG 21-2-0107 20x 
IgG 21-2-0108 20x 
IgG 21-2-0109 20x 
IgG 21-2-0110 20x 
IgG 21-2-0111 20x 
IgG 21-2-0111 20x
