SAMPLES:
All the samples are from Lleida patient’s sera
22nd: 112, 114, 115, 117, 118, 120, 121, 126, 127, 129, control +, control –
23rd: 134, 140, 142, 145, 146, 147, 148, 152, 153, 156, 161, 162, 163, 174, 177, 180, 182, 183, 186, 195, control +, control –
24th: 202, 205, 270, 284, 301, 313, 329, 336, 337, 359, 365, 368, 369, 377, 380, 381, 386, 389, 391, 393, control +, control –
27th: 396, 405, control +, control –
ANO2 ICC:
- HEKs 293 Cells Transfection
- Blocking solution: Goat Serum 5 % in PBS.
- Patient’s sera diluted 1/100
- Primary antibody: ANO2 (TA316534 OriGene) diluted 1/500.
- Secondary antibodies: GAR 488 + GAH 594 IgG (1/1000)
- Vectashield mounting medium.
RESULTS:
All the samples were negative except the positive controls that were positive
