SAMPLES:
All the samples are from Lleida patient’s sera
13th: 17,19, 21, control +, control –
14th: 22, 23, 24, 25, 26, 29, 30, 32, control +, control –
15th: 33, 34, 35, 36, 37, 39, 40, 42, 45, 48, 49, 50, 53, 57, 58, 59, 60, 63, 65, 66, control +, control –
16th: 67, 70, 71, 72, 73, 74, 75, 76, 77, 79, 80, 88, 90, 108, 111, 112, 114, 115, 117, control +, control –
17th: I had to repeat samples 80, 88, 90, 93, 108, 111 because the fluorescence was not clear.
ANO2 ICC:
- HEKs 293 Cells Transfection
- Blocking solution: Goat Serum 5 % in PBS.
- Patient’s sera diluted 1/100
- Primary antibody: ANO2 (TA316534 OriGene) diluted 1/500.
- Secondary antibodies: GAR 488 + GAH 594 IgG (1/1000)
- Vectashield mounting medium.
RESULTS:
All the samples were negative except the positive controls that were positive
