PROCEDURE:
- 900uL of acetone at -20ºC for each 100uL of sera
- Vortex
- Store 1h at -20ºC
- Spin for 10 min at 13.000g (4ºC)
- Eliminate the supernatant
- Mix the pellet with 500uL of acetone at -20ºC
- Spin for 10 min at 13.000g
- Aspirate and left the eppendorf opened for 30min to evaporate acetone.
- Mix with RIPA and proteasa inhibitors
- Incubate 30 min in ice + vortex
- Spin for 10 min at 13.000g
- The supernatant is what we needed, if something precipitate we have to remove it.
- Quantify the protein.
