20200107: ICC FLOT1/2 kit Euroimmun (controls)

SAMPLES:

• First group:
  • 24) CMT 10
  • 25) Positive control MS 163

• Second group:
  • CMT database: CMT 20 – CMT 25
  • IGOS database (SGB): 1 – 19 (except 14)
  • Positive control: MS 163

PROCEDURE

• Patient’s sera diluted 1/10 in PBS-Tween 0,2% (LCR 1/5) –> add 30 microl to each reaction field in the reagent tray: place the biochip slides on them and incubate for 30 min at RT
• Rinse the biochips with a flush of PBS-Tween 0,2% and immerse them in a cuvette containing PBS-Tween 0,2% for 5 minutes.
• Add 25 microL of the fluorescein-labelled secondary antibody (kit) in each reaction field: place the biochip slides on them and incubate for 30 min at RT
• Rinse the biochips with a flush of PBS-Tween 0,2% and immerse them in a cuvette containing PBS-Tween 0,2% for 5 minutes.
• Mount with the mounting medium from the kit

RESULT

All the samples were negative