SAMPLES: 19-198, 19-199, 19-200, ATR plasma (2012), negative control, positive control.
CNTN1 ICC:
- Blocking solution: Rabbit serum diluted 1/40 in PBS.
- Patient’s sera diluted 1/100 for IgG with rabbit serum 1/40.
- Primary antibody: AF904 diluted 1/1000.
- Secondary antibodies: RAG 488 + RAH 594 IgM (1/1000)
- Vectashield mounting medium.
RESULTS: All samples were negative.
