20190926: ICC CNTN1/Caspr1 in CIDP patients

SAMPLES:

1. Vieira (for photo)
2. 19003 (for photo)
3. 19186 (test)
4. 19196 (test)
5. 19197 (test)
6. negative control

CNTN1/Caspr1 co-transfected cells:

• Blocking solution: rabbit serum 1:40 in PBS
• Patients’ sera diluted 1:100 (rabbit serum 1:40 in PBS)
• Ac anti-CNTN1 diluted 1/1000
• RAH 594 IgG and RAG 488 IgG diluted 1/1000
• Vectashield mounting medium

RESULTS: 

1. Vieira (for photo) –> POSITIVE
2. 19003 (for photo) –> POSITIVE
3. 19186 (test) –> POSITIVE
4. 19196 (test) –> NEGATIVE
5. 19197 (test) –> NEGATIVE
6. negative control –> NEGATIVE