Objective: To study anti-CNTN1/Caspr1 Ab titers in CIDP patients
Samples: negative control, MRVM 2011, 17595, 16047, 18859, 19003, Vieira, Piffrei, Regine
Protocol: ICC CNTN1+Caspr1 transfected HEK cells
- Blocking solution: Rabbit serum 1/40 in PBS
- Patient’s sera dilutions in rabbit serum 1/40 in PBS: 1/100, 1/200, 1/400, 1/800, 1/1600, 1/3200, 1/6400
- Primary antibody: AF904 diluted 1/1000
- Secondary antibodies: RAG 488 + RAH 594 (1/1000)
- Vectashield mounting medium
Results:
MRVM 2011, 1:1600
17595, 1:6400
16047, 1:3200
18859, 1:1600
19003, 1:6400
Vieira, 1:1600
Piffrei, 1:6400
Regine, 1:6400
