20190423: ICC NF140 andNF186 IgG in CMT patients (Santander and La Fe)

Samples: 80-105, positive control, negative control.

ICC NF140/NF186:

• Transfection date: 2019.03.12
• Blocking solution: 5% goat serum in PBS.
• Patient’s sera diluted 1/100 with 5% goat serum in PBS.
• Primary antibody: AF3235 diluted 1/1000.
• Secondary antibodies: GAC488 + GAH 594 IgM (1/1000).
• Vectashield mounting medium

RESULTS: All samples were negative. We have to repeat sample 101 for NF140.