Ojective: To test CMT patients for IgG positivity towards NF155 or NF186.
Samples: 58-79, negative control, positive control.
ICC NF140/NF186:
- Transfection date: 2019.03.12
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/100 with 5% goat serum in PBS.
- Primary antibody: AF3235 diluted 1/1000.
- Secondary antibodies: GAC488 + GAH 594 IgM (1/1000).
- Vectashield mounting medium
RESULTS:
All samples were negative.
