Objective: To test CMT patients for IgG positivity towards NF155.
Samples: 86-109, ctrl -, ctrl +
ICC NF155
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/100 for IgG with 5% goat serum in PBS.
- Primary antibody: AF3235 diluted 1/1000.
- Secondary antibodies: GAC488 + GAH 594 IgM (1/1000).
- Vectashield mounting medium.
RESULTS:
All samples were negative, except sample 101 (it has a doubtful staining) –> We are going to do ELISA NF155 IgG.
