Objective: Detection of IgG positivity towards CNTN-1/CASPR-1 in GBS patients from IGOS
Samples: 74-102, CTRL + (17-595), CTRL –
ICC CNTN-1/CASPR-1:
- Blocking solution: Goat serum diluted at 5% in PBS.
- Patient’s sera diluted 1/100 for IgG with goat serum 5% in PBS.
- Primary antibody: Ab 133634 diluted 1/250
- Secondary antibodies: GAR488 + GAH 594 IgG (1/1000)
- Vectashield mounting medium
RESULTS: All the samples were negative.