Detection of IgG positivity towards NF186 in CIDP patients from Bochum
Samples:
- CIDP: 1-34
- negative control
- positive control
Protocol:
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/100 for IgG with 5% goat serum in PBS.
- Primary antibody: AF3235 diluted 1/1000.
- Secondary antibodies: GAC488 + GAH 594 IgM (1/1000).
- Vectashield mounting medium 4µl/Coverslip
Results:
- CIDP1 negative
- CIDP2 negative
- CIDP3 negative
- CIDP5 negative
- CIDP6 negative
- CIDP7 negative
- CIDP9 negative
- CIDP10 negative
- CIDP11 negative
- CIDP12 negative
- CIDP13 negative
- CIDP14 negative
- CIDP15 negative
- CIDP16 negative
- CIDP15 negative
- CIDP16 negative
- CIDP20 negative
- CIDP21 negative
- CIDP22 negative
- CIDP23 negative
- CIDP25 negative
- CIDP26 negative
- CIDP27 negative
- CIDP28 negative
- CIDP29 negative
- CIDP30 negative
- CIDP31 negative
- CIDP32 negative
- CIDP33 negative
- CIDP34 negative
