Objective:
Detection of IgG positivity towards CNTN-1 AND CNTN-1/CASPR-1 in CIDP patients from Kuala Lumpur and one CIDP patient from our hospital (18-885)
SAMPLES: 18-875, 18-876, 18-877, 18-878, 18-879, 18-880, 18-881, 18-885
Negative control
Positive control CNTN-1: 15-198
Positve control CNTN-1/CASPR-1: 17-595
ICC CNTN-1:
- Blocking solution: Rabbit serum diluted 1/40 in PBS.
- Patient’s sera diluted 1/100 for IgG with rabbit serum 1/40.
- Primary antibody: AF904 diluted 1/1000.
- Secondary antibodies: RAG 488 + RAH 594 IgM (1/1000)
- Vectashield mounting medium.
ICC CNTN-1/CASPR-1:
- Blocking solution: Goat serum diluted at 5% in PBS.
- Patient’s sera diluted 1/100 for IgG with goat serum 5% in PBS.
- Primary antibody: Ab 1336341 diluted 1/250
- Secondary antibodies: GAR488 + GAH 594 IgG (1/1000)
- Vectashield mounting medium
RESULTS: All samples were negative except for postive controls
