Objective: To check crossreactivity in an MS sample (279) that seemed to be slightly positive for ANO2.
Samples: 279, CTRL – 2, CTRL + (217).
ANO2 ICC:
- CONDITION 1:
- HEKs 293 Cells Transfection: 14.12.18
- Blocking solution: Goat Serum 5 % in PBS.
- Patient’s sera diluted 1/100
- Primary antibody: ANO2 (TA316534 OriGene) diluted 1/500.
- Secondary antibodies: GAR 488 + GAH 594 IgG (1/1000)
- Vectashield mounting medium.
- CONDITION 2:
- HEKs 293 Cells Transfection: 14.12.18
- Blocking solution: Goat Serum 5 % in PBS.
- Patient’s sera diluted 1/100
- Secondary antibodies: GAH 594 IgG (1/1000)
- Vectashield mounting medium.
RESULTS: The sample was negative.
