Objective:
- Detection of IgG positivity towards NF155/NF186/NF140 in CIDP patients from Kuala Lumpur and one CIDP patient from our hospital.
- To test for IgG positivity towards NF140 in a GBS patients from the spanish cohort of IGOS that seems to be positive for NF140 in ELISA.
SAMPLES:
| SAMPLE | NF155 | NF140 | NF186 | DISEASE |
|---|---|---|---|---|
| 18-871 | - | - | - | CIDP (Sant Pau) |
| 18-875 | + | - | - | CIDP (Kuala Lumpur) |
| 18-876 | - | - | - | CIDP (Kuala Lumpur) |
| 18-877 | - | - | - | CIDP (Kuala Lumpur) |
| 18-878 | - | - | - | CIDP (Kuala Lumpur) |
| 18-879 | - | - | - | CIDP (Kuala Lumpur) |
| 18-880 | - | - | - | CIDP (Kuala Lumpur) |
| 18-881 | + | - | - | CIDP (Kuala Lumpur) |
| 97 | - | - | - | GBS |
| CTRL - 2 | - | - | - | CIDP |
| CTRL + | + | + | + | CIDP |
NF155, NF186 and NF140 ICC:
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/100 for IgG with 5% goat serum in PBS.
- Primary antibody: AF3235 diluted 1/1000.
- Secondary antibodies: GAC488 + GAH 594 IgM (1/1000).
- Vectashield mounting medium
RESULTS: Samples 18-875 and 18-881 were positive for IgG towards NF155. The rest of the samples were negative.
