20181219: ICC ANO2 Multiple Sclerosis

Samples: 300, 301, 302, 305, 306, 307, 309, 311, 312, 315, 318, 322, 323, 324, 325, 326, 327, 317= positive control, negative control

ANO2 ICC:

• HEKs 293 Cells Transfection: 11.12.18
• Blocking solution: Goat Serum 5 % in PBS.
• Patient’s sera diluted 1/100
• Primary antibody: ANO2 (TA316534 OriGene) diluted 1/500.
• Secondary antibodies: GAR 488 + GAH 594 IgG (1/1000)
• Vectashield mounting medium.

RESULTS:

All samples were negative, except the positive control that was positive