Samples (from MS database): 239-265, 269, 274-275, 277-279, 281, 283, 288-295, 297-302, CTRL –
FLOT1/2 ICC:
- HEKs 293 Cells Transfection: 11.12.18
- Blocking solution: Goat Serum 5 % in PBS.
- Patient’s sera diluted 1/100
- Primary antibody: anti-FLOT1 (F1180 Sigma) diluted 1/100.
- Secondary antibodies: GAR 488 + GAH 594 IgG (1/1000)
- Vectashield mounting medium.
RESULTS: some samples are doubtful positive. Maybe there are crossreactivity with the secondary antibodies.
