Samples for AXL:
- ZN015 (IgG)
- ZN002 (IgM)
- ZN020 (IgM)
- ZN061 (IgM)
- Negative control (IgG)
- Negative control (IgM)
Samples for ALCAM:
| 1. 003 (IgG) | 7. ZN009 (IgM) |
| 2. ZN061 (IgG) | 8. ZN058 (IgM) |
| 3. ZN004 (IgM) | 9. ZN012 (IgM) |
| 4. ZN022 (IgM) | 10. ZN061 (IgM) |
| 5. ZN010 (IgM) | 11. Negative control (IgG) |
| 6. ZN026 (IgM) | 12. Negative control (IgM) |
ICC:
- HEKs 293 Cells Transfection: 2018.11.30
- Permeabilize with triton 0’3 % 5 minutes
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/100 for IgG or 1/40 for IgM with 5% goat serum in PBS.
- Primary antibody: c-myc diluted 1/200.
- Secondary antibodies: GAM 488 + GAH 594 IgG (or IgM) (1/1000)
- Vectashield mounting medium.
Results: all the samples are negatives
